Delivery of the Cre recombinase by a self-deleting lentiviral vector: efficient gene targeting in vivo.

摘要: The Cre recombinase (Cre) from bacteriophage P1 is an important tool for genetic engineering in mammalian cells. We constructed lentiviral vectors that efficiently deliver vitro and vivo. Surprisingly, we found a significant reduction proliferation accumulation the G(2)/M phase of Cre-expressing To minimize toxic effect Cre, designed vector integrates into host genome, expresses target cell, subsequently deleted genome Cre-dependent manner. Thus, activity terminates its own expression (self-deleting). showed efficient modification genes brain after transduction with self-deleting vectors. In contrast to sustained expression, transient induced significantly less cytotoxicity. Such promising induction conditional gene modifications minimal toxicity