Oxidized low-density lipoprotein increases interleukin-8 production in human gingival epithelial cell line Ca9-22.
作者： K. Suzuki , Y. Sakiyama , M. Usui , T. Obama , R. Kato
DOI: 10.1111/J.1600-0765.2009.01263.X
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摘要: Suzuki K, Sakiyama Y, Usui M, Obama T, Kato R, Itabe H, Yamamoto M. Oxidized low-density lipoprotein increases interleukin-8 production in human gingival epithelial cell line Ca9-22. J Periodont Res 2010; 45: 488–495. © 2010 John Wiley & Sons A/S Background and Objective: Recent epidemiological studies have shown a correlation between periodontitis hyperlipidemia. We found high levels of oxidized (OxLDL) the crevicular fluid dental patients. In present study, we tried to examine possible role OxLDL periodontal inflammation vitro. Material Methods: Cells Ca9-22 were cultured media containing OxLDL, amounts (IL-8) prostaglandin E2 (PGE2) produced measured using ELISAs. Results: Production IL-8 by cells was significantly increased when treated with but not native LDL or acetylated LDL. PGE2 enhanced co-incubation interleukin-1β (IL-1β). Scavenger receptor inhibitors, fucoidan dextran sulfate, inhibited OxLDL-induced presence IL-1β. The p38 MAPK inhibitors SB203580 SB202190 ERK inhibitor PD98059 production. Among lipids chemically modified LDL, 7-ketocholesterol production. Conclusion: This is first report show that enhances cells.
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