Harnessing glycosylation to improve cellulase activity.

作者: Gregg T Beckham , Ziyu Dai , James F Matthews , Michelle Momany , Christina M Payne

DOI: 10.1016/J.COPBIO.2011.11.030

关键词:

摘要: … enzyme structure and function after secretion. We posit that glycosylation, instead of hindering cellulase engineering, can be used as an additional tool to enhance enzyme activity, …

参考文章(51)
Masatoshi GOTO, Protein O-Glycosylation in Fungi: Diverse Structures and Multiple Functions Bioscience, Biotechnology, and Biochemistry. ,vol. 71, pp. 1415- 1427 ,(2007) , 10.1271/BBB.70080
Alisdair B. Boraston, Linda E. Sandercock, R. Antony J. Warren, Douglas G. Kilburn, O-glycosylation of a recombinant carbohydrate-binding module mutant secreted by Pichia pastoris. Journal of Molecular Microbiology and Biotechnology. ,vol. 5, pp. 29- 36 ,(2003) , 10.1159/000068721
David B Wilson, Cellulases and biofuels. Current Opinion in Biotechnology. ,vol. 20, pp. 295- 299 ,(2009) , 10.1016/J.COPBIO.2009.05.007
M. Lommel, S. Strahl, Protein O-mannosylation: conserved from bacteria to humans. Glycobiology. ,vol. 19, pp. 816- 828 ,(2009) , 10.1093/GLYCOB/CWP066
M. Linder, T. T. Teeri, The cellulose-binding domain of the major cellobiohydrolase of Trichoderma reesei exhibits true reversibility and a high exchange rate on crystalline cellulose. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 93, pp. 12251- 12255 ,(1996) , 10.1073/PNAS.93.22.12251
M. E. Himmel, S.-Y. Ding, D. K. Johnson, W. S. Adney, M. R. Nimlos, J. W. Brady, T. D. Foust, Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science. ,vol. 315, pp. 804- 807 ,(2007) , 10.1126/SCIENCE.1137016
Ingeborg Stals, Bart Samyn, Kjell Sergeant, Theresa White, Katleen Hoorelbeke, An Coorevits, Bart Devreese, Marc Claeyssens, Kathleen Piens, Identification of a gene coding for a deglycosylating enzyme in Hypocrea jecorina. Fems Microbiology Letters. ,vol. 303, pp. 9- 17 ,(2010) , 10.1111/J.1574-6968.2009.01849.X
Steven Geysens, Tiina Pakula, Jaana Uusitalo, Isabelle Dewerte, Merja Penttilä, Roland Contreras, Cloning and Characterization of the Glucosidase II Alpha Subunit Gene of Trichoderma reesei: a Frameshift Mutation Results in the Aberrant Glycosylation Profile of the Hypercellulolytic Strain Rut-C30 Applied and Environmental Microbiology. ,vol. 71, pp. 2910- 2924 ,(2005) , 10.1128/AEM.71.6.2910-2924.2005
Gregg T Beckham, James F Matthews, Yannick J Bomble, Lintao Bu, William S Adney, Michael E Himmel, Mark R Nimlos, Michael F Crowley, None, Identification of amino acids responsible for processivity in a Family 1 carbohydrate-binding module from a fungal cellulase. Journal of Physical Chemistry B. ,vol. 114, pp. 1447- 1453 ,(2010) , 10.1021/JP908810A
Lintao Bu, Gregg T Beckham, Michael R Shirts, Mark R Nimlos, William S Adney, Michael E Himmel, Michael F Crowley, None, Probing Carbohydrate Product Expulsion from a Processive Cellulase with Multiple Absolute Binding Free Energy Methods Journal of Biological Chemistry. ,vol. 286, pp. 18161- 18169 ,(2011) , 10.1074/JBC.M110.212076