Enzymatic synthesis of ligand-bearing DNAs for metal-mediated base pairing utilising a template-independent polymerase
作者： Teruki Kobayashi , Yusuke Takezawa , Akira Sakamoto , Mitsuhiko Shionoya
DOI: 10.1039/C5CC10039A
关键词:
摘要: We have developed a novel method to synthesise artificial ligand-bearing DNAs utilising template-independent DNA polymerase. Hydroxypyridone nucleotides (H) were successively appended primers by the enzyme. The resulting strands, tailed with H nucleotides, formed CuII-mediated metallo-DNA duplexes through formation of metal-mediated base pairs (H–CuII–H).
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