Perturbation factors in the clinical handling of a fiber-coupled Raman probe for cutaneous in vivo diagnostic Raman spectroscopy.

摘要: The application of fiber-coupled Raman probes for the discrimination cancerous and normal skin has advantage a non-invasive in vivo application, easy clinical handling, access to majority body sites, which would otherwise be limited by stationary microscopes. Nevertheless, including optical fibers miniaturizing components, as well measuring vivo, involves sensibility external perturbation factors that could introduce artifacts acquired spectra thereby potentially reduce classification performance. In this study, typical measurements with fiber probe, optimized cancer, were investigated experimentally. Measurements performed under standardized conditions settings on human skin, ex porcine ears. analyzed fingerprint region between 1150 1730 cm(-1) using principal component analysis. largest found influence strong ambient light after miscellaneous pre-treatments such use permanent marker or sunscreen. Minor influences also H2O immersion when varying probe contact force. effect reasonable variation fiber-bending radius was negligible impact. hairy sun-exposed inter-subject variation, investigated. presented results may serve guide avoid negative effects during process data acquisition so misclassification tumor discrimination.