Synergistic anti-oral cancer effects of UVC and methanolic extracts of Cryptocarya concinna roots via apoptosis, oxidative stress and DNA damage.

摘要: Purpose Radiation combined with natural products may improve the radiosensitivity of cancer cells. This study investigated potential a modality treatment Ultraviolet C (UVC; wavelength range 200-280 nm) and our previously identified anti-oral agent (methanolic extracts Cryptocarya concinna roots; MECCrt) in oral Materials methods The mechanism possible synergy UVC MECCrt was explored terms cell viability, cycle, apoptosis, reactive oxygen species (ROS), mitochondrial membrane (MitoMP), DNA damage analyses. Results In viability (%) at 24 h treatment, low doses (14 J/m(2)) (10 μg/ml) resulted slight to human Ca9-22 cells (83.2 80.4) but less harmful normal HGF-1 (93.4 91.8, respectively). (UVC/MECCrt) had lower (54.5%) than or alone no showed significant change cells, expression flow cytometry-based apoptosis (sub-G1 phase, annexin V, pancaspase assays) significantly higher UVC/MECCrt (p < 0.0001). Using cytometry, intracellular ROS levels were for alone. MitoMP H2A histone family member X (γH2AX; H2AFX)-based synergistically inhibited induced by MECCrt/UVC compared its single respectively. Conclusion plus selective killing synergistic anti-proliferative effects against involving oxidative stress, damage. combination therapy appears have great clinical