Ecological Characterisation of the Colonic Microbiota in Arctic and Sub-Arctic Seals
作者： Trine Glad , Vibeke Fam Kristiansen , Kaare M Nielsen , Lorenzo Brusetti , André-Denis G Wright
DOI: 10.1007/S00248-010-9690-X
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摘要: Dominant colonic bacteria in wild hooded (n = 9), harbour 1) and grey seals were identified using 16S rRNA gene clone libraries (313 clones), revealing 52.7% Bacteroidetes, 41.5% Firmicutes, 4.5% Proteobacteria 1.0% Fusobacteria. Thirty (77%) of the 39 phylotypes novel, showing <97% sequence similarity to their nearest cultivated relatives. Mean bacterial cell density, determined by real-time PCR, was high (12.8 log10 cells/g wet wt) for seals, while number methanogenic Archea low (4.0 wt). The level ampicillin (ampr) tetracycline-resistant (tetr) isolates investigated cultivation. Aerobic ampr only detected colon contents from four whereas aerobic tetr found seven nine seals. These data provide novel insight gut microbiota Arctic sub-Arctic living wild.
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