A new heterologous fibrin sealant as scaffold to recombinant human bone morphogenetic protein-2 (rhBMP-2) and natural latex proteins for the repair of tibial bone defects

摘要: Tissue engineering has special interest in bone tissue aiming at future medical applications Studies have focused on recombinant human morphogenetic protein-2 (rhBMP-2) and natural latex proteins due to the osteogenic properties of rhBMP-2 angiogenic characteristic fraction 1 protein (P-1) extracted from rubber tree Hevea brasiliensis. Furthermore, heterologous fibrin sealant (FS) been shown as a promising alternative regenerative therapies. The aim this study was evaluate these substances for repair defects rats. A defect measuring 3 mm diameter created proximal metaphysis left tibia 60 rats implanted with or P-1 combination new FS derived snake venom. animals were divided into six groups: control (unfilled defect), (defect filled 5 μg rhBMP-2), P-1), 8 FS), FS/rhBMP-2 FS/P-1 P-1). sacrificed 2 6 weeks after surgery. newly formed projected margins original exhibited trabecular morphology disorganized arrangement osteocyte lacunae. Immunohistochemical analysis showed intense expression osteocalcin all groups. Histometric revealed significant difference groups (p 0.05). statistically < 0.05) observed relation volume surgical area. In conclusion, found be biocompatible highest osteoconductive capacity healing. These findings suggest application regeneration