3D-bioprinting a genetically inspired cartilage scaffold with GDF5-conjugated BMSC-laden hydrogel and polymer for cartilage repair.
作者： Ye Sun , Yongqing You , Wenbo Jiang , Zanjin Zhai , Kerong Dai
DOI: 10.7150/THNO.38061
关键词:
摘要: Rationale: Articular cartilage injury is extremely common in congenital joint dysplasia patients. Genetic studies have identified Growth differentiation factor 5 (GDF5) as a shared gene and OA progression across different populations. However, few employed GDF5 biological regeneration for articular repair. Methods & Results: In the present study, we report genetic association between loci hip with genome-wide study (GWAS). GWAS replication separate populations achieved significant signals loci. expression was dysregulated allelic differences of DDH upregulated repaired rabbit defect model. vitro enhanced chondrogenesis migration bone marrow stem cells (BMSCs), tested ectopic generation BMSCs by nude mice vivo. Genetically inspired, further generated functional knee construct repair 3d-bioprinting GDF5-conjugated BMSC-laden scaffold. scaffold showed better repairing effects compared to control. Meanwhile, transplantation 3D-bioprinted knees conferred long-term chondroprotection. Conclusions: conclusion, combined replications, which inspired us generate ready-to-implant one-step
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