Functional polymorphisms of the lncRNA H19 promoter region contribute to the cancer risk and clinical outcomes in advanced colorectal cancer

摘要: The long non-coding RNA H19 plays critical roles in cancer occurrence, development, and progression. present study is for the first time to evaluate association of genetic variations promoter region with advanced colorectal (CRC) susceptibility, environmental factors, clinical outcomes. 16 single-nucleotide polymorphisms (SNPs) were identified gene by DNA sequencing, 3 SNPs among which including rs4930101, rs11042170, rs2735970 further expanded samples 572 CRC patients 555 healthy controls. We found that harboring SNP [rs4930101 (P = 0.009), (P = 0.003), rs11042170 (P = 0.003)] or carrying more than one combined risk genotypes significantly increased [P   1 vs. ≤ 1) family history demonstrated significant interactions. Furthermore, a remarkably worse outcome was (> 1 vs. ≤ 1), especially body weight ≥ 61 kg, smoking, first-degree (Log-rank test: P = 0.006, P = 0.018, P = 0.013, respectively). More importantly, multivariate Cox regression analyses verified genotypes > 1 showed prognostic factor weight ≥ 61 kg (P = 0.002), smoking (P = 0.008), (P = 0.006). In addition, MDR analysis consistently revealed combination selected nine known factors better prediction prognosis represented best model predict prognosis. rs27359703 risk. had impact on outcomes ever-smoking, cancer. These data suggest SNPs, these might be potentially functional biomarkers