THE INFLUENCE OF ENVIRONMENTAL TEMPERATURE AND SUBSTRATE INITIAL MOISTURE CONTENT ON ASPERGILLUS NIGER GROWTH AND PHYTASE PRODUCTION IN SOLID-STATE CULTIVATION

摘要: Aspergillus niger is being used commercially for phytase production utilizing solid-state cultivation; however, no studies have been published that investigated the optimal environmental temperature and initial substrate water content to maximize fungal growth and/or production. Solid-state cultivations of on wheat bran soybean meal were conducted at three temperatures (25°C, 30°C, 35°C) moisture contents (50%, 55%, 60% wet basis) in a split-plot full-factorial experimental design. Fermentations 0, 24, 48, 72, 120 h. The containers sampled destructively assayed activity glucosamine concentration as an estimate of fungal biomass. Temperature affected production, but did not. The highest phytase was found 50% to 60% content, 72 h fermentation. Initial moisture content after maximum produced at 35°C, either or results show biomass growth conditions are not same conditions, suggesting entirely correlated with growth.