Changes in the sugar content of barley anthers during culture on different carbohydrates

摘要: The quantity of glucose liberated by an anther homogenate from nine different carbon substrates, was examined to elucidate the rate carbohydrate breakdown tissues in culture. possible relationship between these results and development culture extraction, HPLC analysis soluble sugars anthers cultured on medium containing one four previously tested substrates. Similarly, subjected three inductive pretreatments, were analysed compared with extracts fresh anthers. most successful source culture, when measured fresh, dry weight gain developing anthers, diglucoses maltose cellobiose. Glucose probably diglucose substrates at a similar that which it could be utilized during microspore derived structures. Pretreatments shown increase content providing pulse sugar start This effect particularly pronounced preincubated mannitol, this type pretreatment found promoting structures (measured weight) subsequently maltose.