Selective removal of IgG from the urine of patients with proteinuria using a polymer coated core–shell magnetic nanoparticle

摘要: Selective removal of highly abundant proteins prior to analysis is an important issue in proteomics. In this study, a core–shell structured magnetic nanocomposite Fe3O4@SiO2@MAsp-HEMA was found be effective and selective adsorbent remove IgG from the urine patients with proteinuria. This novel prepared by coating poly(N-methacryloyl-L-aspartic acid-hydroxyethyl methacrylate) shell on Fe3O4@SiO2 nanoparticles via coupling agent KH-570 using mini-emulsion polymerization method. The as-synthesized nanoparticle characterized X-ray diffraction, elemental analysis, thermal gravimetric infrared spectroscopy, hysteresis loop curves, scanning electronic microscopy transmission electron microscopy. A kinetic adsorption experiment performed investigate speed time reach balance. 1D SDS-PAGE capillary electrophoresis were used validate its ability. resulting showed uniform spherical shape at nanoscale dimensions about 200–300 nm, removing efficiency above 80%, maximum capacity 18.08 μg mg−1 15 ± 0.5 °C. binding between ligand confirmed isothermal titration calorimetry. hydrogen bonding force demonstrated as one major interactions functional groups molecular docking experiment.