Plant and fungal invertases in grape berries infected with Botrytis cinerea
作者： H.P. Ruffner , M. Geissmann , D.M. Rast
DOI: 10.1016/0885-5765(92)90059-5
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摘要: Abstract Acid invertases (β-fructofuranosidases) from berries of Vitis vinifera and axenically grown Botrytis cinerea were found to differ substantially in their chromatographic behaviour on Concanavalin A-Sepharose. While the partially purified plant enzyme showed low affinity for lectin desorbed readily 0·1 m methylmannoside solution, interaction fungal invertase was stable desorption slow even at 1 eluant concentrations. Based this criterion, samples both organisms mixed, extracted, enzymes re-separated point where individual assessments activities possible. The recovery factors subsequently used quantitate vivo contributions sucrose cleavage immature diseased grapes, extracted week after infection with B. . Total determined 20 g (fresh weight) material presporulating stage infection. They amounted approximately 400 nkat grape about 150 invertase. role relative attributed host or pathogen is discussed relation type phytopathogenic association hand.
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