Effective combination of hydrostatic pressure and aligned nanofibrous scaffolds on human bladder smooth muscle cells: implication for bladder tissue engineering

摘要: Bladder tissue engineering has been the focus of many studies due to its highly therapeutic potential. In this regard aspects such as biochemical and biomechanical factors need be studied extensively. Mechanical stimulations hydrostatic pressure topology matrices are critical features which affect normal functions cells involved in bladder regeneration. study, (10 cm H2O) stretch forces were exerted on human smooth muscle (hBSMCs) seeded aligned nanofibrous polycaprolactone/PLLA scaffolds, alterations gene protein expressions studied. The transcription patterns for collagen type I, III, IV, elastin, α-SMA, calponin caldesmon monitored days 3 5 quantitatively. Changes desmin, I III quantified by Enzyme-linked immuno-sorbent assay. scaffolds characterized using scanning electron microscope, contact angle measurement tensile testing. positive effect mechanical functional improvement engineered was supported translational down-regulation α-SMA VWF, up-regulation desmin III:I ratio. Altogether, our study reveals that proper combination with appropriate surface stimulation hBSMCs causes a tissue-specific phenotype needs considered engineering.