Macrophage-inducedEnhancementof EndogenousTumor LysosomeActivity1

摘要: Activated macrophages have the capacity to selectively in jure neoplastic cells. Morphological observations by others suggest that final effectors of macrophage-mediated tumor cytotoxicity are lysosomes activated macrophage origin which translocated directly into susceptible target The purpose this study was quantitatively determine if elevated levels specific lysosomal activity present cells exposed vitro. Effector were obtained from peritoneal cavities A/ BAŒF/F50+and C3H/HeN(MTV~) glucan-treated mice mammary virus is negative. Target tumorigenic and nontumorigenic fibroblast cell lines derived same two strains. Macrophage-dependent cytotox icity quantitated using [3H]thymidine incorporation inhibi tion 51Crpostlabeling assays. Tumor lysosome newly developed microspectrophotometric assays for hydrolase acid phosphatase lysosomotropic probe acridine orange. results dem onstrate correlates with degree generated effector macrophages. Interestingly, macrophage-induced elevation does not appear represent acquisition macrophage-derived organelles; rather, it appears an increase number or size intact, endogenous due macrophage-dependent reduction density. This finding suggests clinically proven growth-reducing regimens such as host activation may be useful adjuncts cancer therapies designed promote labilize lysosomes.