An acyl-coenzyme A dehydrogenase assay utilizing the ferricenium ion.
作者： Thomas C. Lehman , Daniel E. Hale , Ajay Bhala , Colin Thorpe
DOI: 10.1016/0003-2697(90)90080-S
关键词:
摘要: A sensitive assay for medium chain acyl-CoA dehydrogenase has been developed by substituting ferricenium hexafluorophosphate the physiological acceptor, electron transferring flavoprotein. The ion is a facile oxidant of octanoyl-CoA-reduced enzyme with Vmax 1400 min-1 and KM 55 microM at pH 7.6. does not require additional mediator dyes, exhibits low background rates, avoids necessity purifying substantial amounts Unlike fluorescence-based flavoprotein assay, this new procedure can be performed aerobically. Both assays give comparable results when tested crude fibroblast homogenates from normal deficient patients. convenience method suggests it may generally useful as screening number dehydrogenases.
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