Genome-Wide Identification of Long Noncoding RNAs in CD8+ T Cells
作者： Ken C. Pang , Marcel E. Dinger , Tim R. Mercer , Lorenzo Malquori , Sean M. Grimmond
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摘要: Previous research into the molecular mechanisms that underlie Ag-specific CD8+ T cell differentiation and function has largely focused on role of proteins. However, it is now apparent mammalian genome expresses large numbers long (>200 nt) nonprotein-coding RNAs (ncRNAs), there increasing evidence these have important regulatory functions, particularly in regulation epigenetic processes underpinning differentiation. In this study, we show cells express hundreds ncRNAs, many which are lymphoid-specific and/or change dynamically with lymphocyte or activation. Numerous ncRNAs surround overlap immunologically protein-coding genes can be predicted to via a range mechanisms. The expressed microRNAs small interfering further suggests may processed exert their effects smaller functional species. Finally, majority harbor signatures evolutionary conservation, secondary structures, regulated promoters, supporting functionality. Taken together, our findings represent first systematic discovery suggest transcripts likely play adaptive immunity.
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Daniela S Gerhard, L Wagner, EA Feingold, CM Shenmen, LH Grouse, G Schuler, SL Klein, S Old, R Rasooly, P Good, M Guyer, AM Peck, JG Derge, D Lipman, FS Collins, W Jang, S Sherry, M Feolo, L Misquitta, E Lee, K Rotmistrovsky, SF Greenhut, CF Schaefer, K Buetow, TI Bonner, D Haussler, J Kent, M Kiekhaus, T Furey, M Brent, C Prange, K Schreiber, N Shapiro, NK Bhat, RF Hopkins, F Hsie, T Driscoll, MB Soares, TL Casavant, TE Scheetz, Brown-stein MJ, TB Usdin, S Toshiyuki, P Carninci, Y Piao, DB Dudekula, MS Ko, K Kawakami, Y Suzuki, S Sugano, CE Gruber, MR Smith, B Simmons, T Moore, R Waterman, SL Johnson, Y Ruan, CL Wei, S Mathavan, PH Gunaratne, J Wu, AM Garcia, SW Hulyk, E Fuh, Y Yuan, A Sneed, C Kowis, A Hodgson, DM Muzny, J McPherson, RA Gibbs, J Fahey, E Helton, M Ketteman, A Madan, S Rodrigues, A Sanchez, M Whiting, A Madari, AC Young, KD Wetherby, SJ Granite, PN Kwong, CP Brinkley, RL Pearson, GG Bouffard, RW Blakesly, ED Green, MC Dickson, AC Rodriguez, J Grimwood, J Schmutz, RM Myers, YS Butterfield, M Griffith, OL Griffith, MI Krzywinski, N Liao, R Morin, R Morrin, D Palmquist, AS Petrescu, U Skalska, DE Smailus, JM Stott, A Schnerch, JE Schein, SJ Jones, RA Holt, A Baross, MA Marra, S Clifton, KA Makowski, S Bosak, J Malek, The status, quality, and expansion of the NIH full-length cDNA project: The Mammalian Gene Collection (MGC) Genome Research. ,vol. 14, pp. 2121- 2127 ,(2004) , 10.1101/GR.2596504
E M Shevach, T R Malek, R J Robb, G Ortega, The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells. Journal of Immunology. ,vol. 133, pp. 1970- 1975 ,(1984)
M O Dailey, S M Rigby, J L Mobley, Regulation of adhesion molecule expression by CD8 T cells in vivo. II. Expression of L-selectin (CD62L) by memory cytolytic T cells responding to minor histocompatibility antigens. Journal of Immunology. ,vol. 153, pp. 5443- 5452 ,(1994)
Tim Willinger, Tom Freeman, Mark Herbert, Hitoshi Hasegawa, Andrew J. McMichael, Margaret F. C. Callan, Human Naive CD8 T Cells Down-Regulate Expression of the WNT Pathway Transcription Factors Lymphoid Enhancer Binding Factor 1 and Transcription Factor 7 (T Cell Factor-1) following Antigen Encounter In Vitro and In Vivo The Journal of Immunology. ,vol. 176, pp. 1439- 1446 ,(2006) , 10.4049/JIMMUNOL.176.3.1439
David P. Bartel, Chang-Zheng Chen, Micromanagers of gene expression: the potentially widespread influence of metazoan microRNAs Nature Reviews Genetics. ,vol. 5, pp. 396- 400 ,(2004) , 10.1038/NRG1328
, Antisense Transcription in the Mammalian Transcriptome Science. ,vol. 309, pp. 1564- 1566 ,(2005) , 10.1126/SCIENCE.1112009
Jonathan W. Yewdell, Nicholas P. Restifo, Jack R. Bennink, Igor Bačík, Laurence C. Eisenlohr, Steven A. Rosenberg, Kari R. Irvine, Barbra J. McCabe, Robert W. Anderson, Antigen processing in vivo and the elicitation of primary CTL responses. Journal of Immunology. ,vol. 154, pp. 4414- 4422 ,(1995)
Elizabeth A. Jones, Richard A. Flavell, Distal enhancer elements transcribe intergenic RNA in the IL-10 family gene cluster. Journal of Immunology. ,vol. 175, pp. 7437- 7446 ,(2005) , 10.4049/JIMMUNOL.175.11.7437
John N Hutchinson, Alexander W Ensminger, Christine M Clemson, Christopher R Lynch, Jeanne B Lawrence, Andrew Chess, A screen for nuclear transcripts identifies two linked noncoding RNAs associated with SC35 splicing domains BMC Genomics. ,vol. 8, pp. 39- 39 ,(2007) , 10.1186/1471-2164-8-39
P Froussard, P Chastagner, W Greene, A Abadie, S Longacre, G Sommé, J Thèze, p55 IL-2 receptor mRNA precursors in murine T lymphocyte nuclei. Journal of Immunology. ,vol. 141, pp. 1358- 1364 ,(1988)