Schistosoma mansoni excretory circulating cathodic antigen shares Lewis-x epitopes with a human granulocyte surface antigen and evokes host antibodies mediating complement-dependent lysis of granulocytes.

摘要: Parasitic worms of the genus Schistosoma excrete relatively large amounts immunogenic glycoproteins (circulating cathodic antigen [CCA]) that contain polysaccharide side chains with trisaccharide Lewis-x (L(ex)) as a repeating unit. These carbohydrates evoke high titers specific IgM antibodies cross-react L(ex) units on surface granulocytes. Consequently this might lead, in presence complement, to lysis In present study, hypothesis was investigated using anti-CCA mouse monoclonal (MoAbs) and polyclonal purified from sera infected humans. By flow cytometry, it demonstrated MoAbs directed against CCA strongly recognized It could also be shown these MoAbs, well human sera, caused granulocytes complement-dependent cytotoxicity assay. Sera healthy controls or patients other helminth infections resulted negligible granulocytotoxicity. vitro observed phenomena may explain mild moderate neutropenia occurs schistosomiasis patients.