Cis-acting sequences in the 5'-untranslated region of the ribosomal protein A1 mRNA mediate its translational regulation during early embryogenesis of Drosophila.
作者： R C Patel , M Jacobs-Lorena
DOI: 10.1016/S0021-9258(18)48409-8
关键词:
摘要: The rate of ribosomal (r)-protein synthesis in the early Drosophila embryo is low despite presence abundant, maternally supplied r-protein mRNAs. This due to specific repression mRNA translation. In contrast mRNAs, most other mRNAs are efficiently translated embryo. Here we report on identification cis-acting sequences that mediate translational A1 (rpA1) mRNA. Chimeric genes containing from translationally regulated rpA1 fused constitutively alpha-tubulin were constructed and transformed into germ line. Translation corresponding hybrid was measured ovaries embryos transgenic flies. results indicated a 89-nucleotide sequence untranslated leader by itself sufficient confer full regulation heterologous
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