Human Uveal Melanoma Cells Produce Macrophage Migration-Inhibitory Factor to Prevent Lysis by NK Cells

摘要: Human uveal melanoma arises in an immune privileged ocular environment which both adaptive and innate effector mechanisms are suppressed. Uveal is the most common intraocular tumor adults derived from tissues eye that produce macrophage migration-inhibitory factor (MIF), a cytokine has recently been demonstrated to immediate inhibition of NK cell-mediated lytic activity. Although lysis melanomas inhibited eye, cells disseminate at risk for surveillance by cells. Moreover, demonstrate propensity metastasize liver, organ with one highest levels activity body. Therefore, we speculated produced MIF as means escaping lysis. Accordingly, seven primary cell lines two metastases were examined their production MIF. was detected culture supernatants ELISA classical bioassay migration inhibition. Melanoma-derived YAC-1 Cell approximately twice much biologically active cultures melanomas. Inhibition killing melanoma-derived specifically dose-dependent manner anti-MIF Ab. The results suggest human maintain microenvironment privilege secreting protects against killing.