Molecular classification of breast phyllodes tumors: validation of the histologic grading scheme and insights into malignant progression

摘要: Phyllodes tumors of the breast are rare fibroepithelial neoplasms with a potential for recurrence. Current histological classification is not always predictive clinical behavior. The aim this study was to identify genetic changes associated development borderline and malignant phyllodes in an Asian population, assess if data supported categorization these into existing three grades benign, borderline, malignant. Expression profiling 21 (6 10 5 malignant) performed using Affymetrix U133Plus 2.0 GeneChips®. Gene expression among compared 29 gene list able classify them according their histologic grade. Among genes those responsible matrix formation, cell adhesion, epidermis proliferation. Comparative genomic microarray analysis showed that most common chromosomal alteration 1q gain, increasing number noted Upregulation HOXB13 seen relative further investigated by immunohistochemistry corresponding set formalin-fixed, paraffin-embedded tumors. protein overexpression found be correlated stromal hypercellularity atypia (P = 0.03, P 0.039, respectively) may implicated