Detection of human papillomavirus genome in nasolaryngeal papillomas using digoxigenin labeled DNA probes

摘要: It is being reported that human papillomavirus (HPV) has been implicated in the pathogenesis of various neoplastic lesions genital organs. To investigate etiological role HPV and its types nasolaryngeal papillomas, we retrospectively analyzed genomes by nucleic acid hybridization methods ; for detecting DNA mRNA, employed recently developed non-radioactive (digoxigenin labeled) probes compared results radioisotope methods.In total, 43 cases papillomatous were examined. They verruca vulgaris nasal vestibule (Nr=2), inverted papilloma (IP, Nr=26), laryngeal (Nr=15).HPV examined type 2, 6, 11, 16 18.Two shown to contain HPV-2 mRNA situ hybridization. HPV-11 was detected 3 (12%) whereas HPV-16 1 case (4%) latter associated with squamous cell carcinoma.These suggest may be development IP, play an important malignant transformation IP. In multiple (Nr=8, one juvenile 7 adult type), either HPV-6 or at high rate (6/8, 75%). The presence provides strong evidence etiology these papillomas. Whereas single (Nr=7), not detected.Technically, sensitivity digoxigenin (DIG) labeled probe almost same as 35S dot blot hybridization, thus applied DIG Southern low background. By using probes, rates detection equal those probes. But non-specific reaction occasionally found DIG-labeled However, pretreatment DNase orRNase markedly diminished binding acids. Thus, DIG-label excellent substitutional method radioisotope.