Knee and hip articular cartilage have distinct epigenomic landscapes: implications for future cartilage regeneration approaches

摘要: Objectives To elucidate the functional epigenomic landscape of articular cartilage in osteoarthritis (OA) affected knee and hip joints relation to gene expression. Methods Using Illumina Infinium HumanMethylation450 BeadChip arrays, genome-wide DNA methylation was measured 31 preserved lesioned sample pairs (14 knees 17 hips) from patients who underwent a total joint replacement due primary OA. previously published expression data 33 samples, which 13 were overlapping with current dataset, we assessed differences differentially methylated regions (DMRs). Results Principal component analysis revealed distinct clustering irrespective OA pathophysiology. A 6272 CpG dinucleotides between two joints, comprising 357 DMRs containing 1817 CpGs 245 unique genes. Enrichment genes proximal significant enrichment for developmental pathways homeobox (HOX) Subsequent transcriptomic DMR exposed patterns. Conclusions Our findings reveal consistent that marked among HOX genes, not reflecting temporal sequential pattern during development. This implies mechanisms maintaining integrity adulthood, thereby contributing our understanding homeostasis future tissue regeneration approaches.