Comparative genomic hybridization of formalin-fixed, paraffin-embedded breast tumors reveals different patterns of chromosomal gains and losses in fibroadenomas and diploid and aneuploid carcinomas
作者： Heidi Holtgreve-Grez , Thomas Ried , Stanislas du Manoir , Thomas Cremer , Evelin Schröck
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摘要: Comparative genomic hybridization serves as a screening test for regions of copy number changes in tumor genomes. We have applied the technique to map DNA gains and losses 33 cases formalin-fixed, paraffin-embedded primary breast tumors (13 fibroadenomas 10 diploid aneuploid carcinomas). No imbalances were found fibroadenomas. Recurrent findings adenocarcinomas include increases chromosomes 1q (14 20 samples), 8q (10 20), 17q (5 6p (3 13q 16p decreases 22 (7 17p (6 20). Regional high level observed on chromosome bands 1q32, 8p11, 8q24, 10p, 11q13, 12p, 12q15, 17q11-12, 17q22-24. The majority samples studied gene amplification c-myc, c-erbB2, cycD1, int-2 by means Southern blot analysis. comparison with ploidy measurements revealed different distribution significantly higher chromosomal aberrations than
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