Proteomic identification of biomarkers in the cerebrospinal fluid (CSF) of astrocytoma patients.

摘要: The monitoring of changes in the protein composition cerebrospinal fluid (CSF) can be used as a sensitive indicator central nervous system (CNS) pathology, yet its systematic application to analysis CNS neoplasia has been limited. There is pressing need for both better understanding gliomagenesis and development reliable biomarkers disease. In this report, we two proteomic techniques, two-dimensional gel electrophoresis (2-DE), cleavable Isotope-Coded Affinity Tag (cICAT) compare CSF proteomes identify tumor- grade-specific patients bearing brain tumors differing histologies grades. Retrospective analyses were performed on 60 samples derived from astrocytomas WHO grade II, III, IV, schwannomas, metastastic tumors, inflammatory samples, non-neoplastic controls. We identified 103 potential tumor-specific markers which 20 high-grade astrocytoma-specific. These investigations allowed us spectrum signature proteins that could distinguish control versus those with low- (AII) or (AIV) astrocytoma. may represent new diagnostic, prognostic, disease follow-up when alone combination. candidate also have functional properties play critical role malignant progression human astrocytomas, thus possibly representing novel therapeutic targets highly lethal