Human fibronectin contains distinct adhesion- and motility-promoting domains for metastatic melanoma cells.

摘要: The active migration of tumor cells through extracellular matrices has been proposed to play a role in certain aspects metastasis. Metastatic migrate vitro response substratum-bound adhesive glycoproteins such as fibronectin. present studies use affinity-purified proteolytic fragments fibronectin determine the nature adhesion- and/or motility-promoting domains within protein. Two distinct were identified with cell adhesion-promoting activities. By number criteria, activity promoted by these two was distinct. One fragment, 75-kD tryptic fragment purified monoclonal antibody chromatography, adhesion, spreading, and haptotactic motility melanoma cells. Experiments using synthetic attachment peptide solution indicated that at least part exhibited is mediated sequence arg-gly-asp-ser. It not possible demonstrate migration-stimulating small (11.5 kD) peptic containing this (Pierschbacher, M.D., E. G. Hayman, Ruoslahti, 1981, Cell, 26:259-267) suggesting another cell-binding 75 kD from arg-gly-asp-ser might be required for motility. second stimulated adhesion 33-kD tryptic/catheptic carboxyl-terminal heparin-binding which localized A chain This promotes spreading but Melanoma sensitive effects cycloheximide, contrasted haptotaxis-promoting fragment. Importantly, illustrate haptotaxis due simple gradients results are discussed light model multiple surface constituents mediating on