STANDARDIZATION OF IN VITRO STERILIZATION AND CALLUS INDUCTION PROTOCOL FOR LEAF EXPLANTS OF ANCHOTE : COCCINIA ABYSSINICA
作者： Tola Bayisa Guma , Kahia Jane , Onguso Justus , Peter Njenga Kariuki , None
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摘要: The present study was conducted to develop efficient sterilization and callus induction protocols of Anchote leaf explants. (Coccinia abyssinica) roots were collected from West Ethiopia transported Kenya where they grown in the net greenhouse Juja. Leaves explants harvested seedlings used for both protocols. Young selected healthy plants subjected using 5 10 % commercial bleach of( NaOCl (3.85%) /(JIK®) )at ,10 15 minutes intervals. Maximum clean survival obtained (82.5±0.5) at 5%NaOC with 10Minutes) . Best maximum induction(80±2) achieved combination 5µm(BAP+2,4-D).
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