MicroRNA-335-5p inhibits osteoblast apoptosis induced by high glucose
作者： JILING LI , ZHENGPING FENG , LIXUE CHEN , XIAOJU WANG , HUACONG DENG
关键词:
摘要: Diabetic osteoporosis represents a serious health condition with increasing incidence. Previous studies have shown that microRNA (miR)-335-5p is highly expressed in MC3T3-E1 osteoblasts and promotes their differentiation via downregulating the expression of dickkopf‑1 (DKK1). The present study investigated effects miR‑335‑5p on apoptosis induced by high glucose (HG), as well underlying molecular mechanisms. MC3T3‑E1 were transfected mimics or control miR cultured under HG conditions for seven days. Reverse‑transcription PCR showed that, compared group, levels significantly downregulated group. However, no significant differences observed mRNA DKK1 between these groups. Furthermore, flow cytometric analysis apoptotic rate was increased >2‑fold group while overexpression decreased model cells ~40%. In addition, western blot revealed protein caspase‑3 elevated which inhibited miR‑335‑5p. These results may indicate HG‑induced through decreasing DKK1. suggested represent potential target treatment diabetic osteoporosis.
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