A Systematic Analysis on mRNA and MicroRNA Expression in Runting and Stunting Chickens

摘要: Runting and stunting syndrome (RSS), which is characterized by lower body weight, widely occurs in broilers. Some RSS chickens simply exhibit slow growth without pathological changes. An increasing number of studies indicate that broiler strains differ susceptibility to infectious diseases, most likely due their genetic differences. The objective this study was detect the differentially expressed miRNAs mRNAs normal chickens. By integrating miRNA with mRNA expression profiling, potential molecular mechanisms involved could be further explored. Twenty-two known 1,159 genes were compared (P < 0.05). qPCR validation results displayed similar patterns. primarily energy metabolism pathways. antisense transcripts extensively chicken liver albeit reduced abundance. Dual-luciferase reporter assay indicated gga-miR-30b/c directly target CARS through binding its 3′UTR. miR-30b/c: regulation mainly occurred liver. In thigh muscle hypothalamus, miR-30b/c are at higher levels from 2 6 w age, notably significant differences observed 4 age.