Diagnostic utility of cell cycle and apoptosis regulatory proteins in verrucous squamous carcinoma.

摘要: A major problem in the diagnosis of verrucous squamous cell carcinoma is lack readily reproducible objective criteria for distinguishing this malignant lesion from reactive epithelial hyperplasia. Both lesions are characterized by thickened (well-differentiated) epithelium without cellular atypia and subjacent stroma densely infiltrated lymphocytes plasma cells. This study was carried out to evaluate use cycle apoptosis-related regulatory proteins carcinoma. The materials consisted representative formalin-fixed paraffin-embedded tissue blocks 19 cases carcinoma, 18 classic 14 hyperplasia (acanthosis). immunohistochemical expression following evaluated using avidin-biotin complex detection technique: p16, p21, p53, Ki67, retinoblastoma gene product (RBGP) (also known as protein [pRb]). Expression Ki67 detected only single basal layer all acanthosis. In suprabasal cells lower third neoplastic (100%). with frankly invasive diffusely expressed throughout entire thickness well underlying tumor nests. pattern p53 similar that experimental groups, a Pearson correlation coefficient 0.98. addition, hyperplastic very weak, contrast more intense immunoreactivity observed There an overlapping RGBP being present than half 50% 100% each group. We therefore conclude distinctly different seen Thus immunostains reliable adjuncts may be helpful resolving diagnostic problems associated