A rapid and sensitive assay for determination of cholesterol in membrane lipid extracts
作者： Peter Ott , Yvonne Binggeli , Urs Brodbeck
DOI: 10.1016/0005-2736(82)90101-8
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摘要: Abstract A commercially available enzymatic assay (Boehringer Monotest) was modified to allow a rapid and sensitive determination of cholesterol in membrane lipid extracts. This achieved by adding 0.5% Triton X-100 the reagent solution. The detergent did not interfere with assay. relationship between amount per absorbance at 500 nm linear up 100 μg. recovery better than 95%. applied erythrocyte
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