Western immunoblot analysis of protein antigens ofPenicillium marneffei

摘要: Protein antigens of Penicillium marneffei prepared during the yeast and mould phases in vitro growth were analyzed by gel electrophoresis immunoblot assay. More than 20 phase proteins detected Coomassie staining; among these, at least 10 reacted with IgG pooled sera 28 AIDS patients penicilliosis. Four immunogenic 200, 88, 54 50 kDa produced large quantity deceleration early stationary growth. When these individual derived from 33 penicilliosis, reactivities to protein 72·7, 93·9, 60·6 57·6%, respectively. The bands gave strong reactions about a half serum samples. In one an patient, could be strongly two months before definite diagnosis fungal culture. components form lower yield and...