Oral ingestion of Lentinula edodes mycelia extract inhibits B16 melanoma growth via mitigation of regulatory T cell-mediated immunosuppression.

摘要: Mitigation of regulatory T cell-mediated immunosuppression is crucial for optimal in vivo anti-tumor immune responses. In this study, we examined the effect induced by oral ingestion an immunomodulating diet comprised Lentinula edodes mycelia (L.E.M.) extract. C57BL/6 mice were inoculated subcutaneously footpad with B16 melanoma and fed L.E.M. Ingestion extract significantly inhibited tumor growth, was not observed nude mice, suggesting a cell-dependent mechanism. addition, led to significant restoration H-2K(b) -restricted melanoma-reactive cells spleen draining lymph nodes melanoma-bearing mice. Flow cytometry analysis revealed that percentage Foxp3(+) CD4(+) increased but decreased Importantly, selective depletion CD8(+) abolished L.E.M.-induced effect, whereas or CD25(+) showed no additive influence on effect. Real-time PCR level Foxp3 mRNA within tissues, lowered plasma transforming growth factor (TGF)-β. Furthermore, vitro assay immunosuppressive activity from canceled Our results indicate restores responses class I-restricted presumably mitigation cells-mediated immunosuppression.