siRNA-mediated knockdown of aryl hydrocarbon receptor nuclear translocator 2 affects hypoxia-inducible factor-1 regulatory signaling and metabolism in human breast cancer cells
作者： Xian-Yang Qin , Feifei Wei , Jun Yoshinaga , Junzo Yonemoto , Masaru Tanokura
DOI: 10.1016/J.FEBSLET.2011.09.017
关键词:
摘要: Recent human studies found that the mRNA expression level of aryl-hydrocarbon receptor nuclear translocator 2 (ARNT2) was positively associated with prognosis breast cancer. In this study, we used small interfering RNA techniques to knockdown ARNT2 in MCF7 cancer cells, and an almost 40% downregulation increased sensitive apoptosis gene (3.36-fold), decreased von Hippel-Lindau (0.27-fold) matrix metalloproteinase-1 (0.35-fold). The metabolite analysis revealed contents glucose, glycine, betaine, phosphocholine, pyruvate lactate involved hypoxia-inducible factor (HIF)-1-dependent glycolytic pathway were significantly lower cells treated siARNT2. Our results suggested might play important role modulation HIF-1-regulated signaling metabolism.
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sci-hub.se 参考文章(31)
Adrian L. Harris, Hypoxia — a key regulatory factor in tumour growth Nature Reviews Cancer. ,vol. 2, pp. 38- 47 ,(2002) , 10.1038/NRC704
M Tan, Q Gu, H He, D Pamarthy, G L Semenza, Y Sun, SAG/ROC2/RBX2 is a HIF-1 target gene that promotes HIF-1α ubiquitination and degradation Oncogene. ,vol. 27, pp. 1404- 1411 ,(2008) , 10.1038/SJ.ONC.1210780
Oliver Fiehn, Metabolomics - the link between genotypes and phenotypes Plant Molecular Biology. ,vol. 48, pp. 155- 171 ,(2002) , 10.1023/A:1013713905833
K Hirose, M Morita, M Ema, J Mimura, H Hamada, H Fujii, Y Saijo, O Gotoh, K Sogawa, Y Fujii-Kuriyama, cDNA cloning and tissue-specific expression of a novel basic helix-loop-helix/PAS factor (Arnt2) with close sequence similarity to the aryl hydrocarbon receptor nuclear translocator (Arnt). Molecular and Cellular Biology. ,vol. 16, pp. 1706- 1713 ,(1996) , 10.1128/MCB.16.4.1706
Oliver Hankinson, Why does ARNT2 behave differently from ARNT Toxicological Sciences. ,vol. 103, pp. 1- 3 ,(2008) , 10.1093/TOXSCI/KFN032
Laura N. Vandenberg, Russ Hauser, Michele Marcus, Nicolas Olea, Wade V. Welshons, Human exposure to bisphenol A (BPA). Reproductive Toxicology. ,vol. 24, pp. 139- 177 ,(2007) , 10.1016/J.REPROTOX.2007.07.010
Muireann Coen, Elaine Holmes, John C. Lindon, Jeremy K. Nicholson, NMR-Based Metabolic Profiling and Metabonomic Approaches to Problems in Molecular Toxicology Chemical Research in Toxicology. ,vol. 21, pp. 9- 27 ,(2008) , 10.1021/TX700335D
O. Iliopoulos, A. P. Levy, C. Jiang, W. G. Kaelin, M. A. Goldberg, Negative regulation of hypoxia-inducible genes by the von Hippel-Lindau protein Proceedings of the National Academy of Sciences of the United States of America. ,vol. 93, pp. 10595- 10599 ,(1996) , 10.1073/PNAS.93.20.10595
Tomonori Hosoya, Yoshihito Oda, Satoru Takahashi, Masanobu Morita, Shimako Kawauchi, Masatsugu Ema, Masayuki Yamamoto, Yoshiaki Fujii-Kuriyama, Defective development of secretory neurones in the hypothalamus of Arnt2-knockout mice Genes to Cells. ,vol. 6, pp. 361- 374 ,(2001) , 10.1046/J.1365-2443.2001.00421.X
Hiroki Sekine, Junsei Mimura, Masayuki Yamamoto, Yoshiaki Fujii-Kuriyama, Unique and overlapping transcriptional roles of arylhydrocarbon receptor nuclear translocator (Arnt) and Arnt2 in xenobiotic and hypoxic responses. Journal of Biological Chemistry. ,vol. 281, pp. 37507- 37516 ,(2006) , 10.1074/JBC.M606910200