Fabrication, ultra-structure characterization and in vitro studies of RF magnetron sputter deposited nano-hydroxyapatite thin films for biomedical applications

摘要: Abstract A series of nanostructured low-crystalline hydroxyapatite (HA) coatings averaging 170, 250, and 440 nm in thickness were deposited onto previously etched titanium substrates through radio-frequency (RF) magnetron sputtering. The HA analyzed using infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning transmission electron microscopy (SEM TEM). Cross sections the thin specimens prepared by FIB to study microstructure TEM. deposition process formed nano-scale grains, generating an amorphous layer at substrate/coating interface inducing growth a columnar grain structure perpendicular substrate surface. microstructural analysis film confirmed that size crystallinity increased when increasing time. not cytotoxic, as proven vitro assays primary dental pulp stem cells mouse fibroblast NCTC clone L929 cells. Low-crystallinity with different thicknesses stimulated attach, proliferate form mineralized nodules on surface better than uncoated substrates.