Establishment of a convenient system for the long-term culture and study of non-neoplastic human salivary gland epithelial cells

摘要: Epithelial cells appear to play an important role in the initiation and maintenance of autoimmune lesions salivary glands patients with Sjogren's syndrome. Therefore, detailed study immunological function gland epithelial (SGEC) may provide useful information for understanding syndrome pathogenesis. In this report we aimed formulate a protocol establishment human non-neoplastic SGEC lines as tool physiology pathophysiology these cells. Pointing towards practical approach, sought establish from quite limited amount biopsy tissue obtained during diagnostic evaluation patients. Herein, favorable conditions long-term are presented involve successive application serum-containing serum-free culture medium, supplemented essential growth factors. This has been found reliable convenient, attested by reproducible lines. The analysis phenotypic features, well coculture system interactions between lymphocytes, also described. Such techniques valuable means functional molecular investigation particularly other disorders glandular epithelia.