Three distinct cell phenotypes of induced‐TNF cytotoxicity and their relationship to apoptosis

摘要: We have identified three distinct cell phenotypes with respect to the conditions under which cells became susceptible TNF-mediated lysis. These include: 1) treatment protein synthesis inhibitor, cycloheximide; 2) contact activated macrophages, and 3) infection vaccinia virus. Whereas virus-infected 3T3 sensitive soluble TNF, F5b required macrophages. showed that "macrophage-resistant" F5m did not become TNF or killing by macrophages after Therefore, does sensitize all TNF. also determined pathways of lysis for sensitization. 3T3, LM929, were killed process necrosis, was characterized release low mol wt DNA fragments (apoptosis).