Mapping Novel Traits by Array-Assisted Bulk Segregant Analysis in Saccharomyces cerevisiae

作者: Matthew J. Brauer , Cheryl M. Christianson , Dave A. Pai , Maitreya J. Dunham

DOI: 10.1534/GENETICS.106.057927

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摘要: We demonstrate a new method, microarray-assisted bulk segregant analysis, for mapping traits in yeast by genotyping pooled segregants. apply probabilistic model to the progeny of single cross and as little two microarray hybridizations reliably map an auxotrophic marker, Mendelian trait, major-effect quantitative trait locus.

参考文章(14)
Samuel P. Hazen, Justin O. Borevitz, Frank G. Harmon, Jose L. Pruneda-Paz, Thomas F. Schultz, Marcelo J. Yanovsky, Sarah J. Liljegren, Joseph R. Ecker, Steve A. Kay, Rapid Array Mapping of Circadian Clock and Developmental Mutations in Arabidopsis Plant Physiology. ,vol. 138, pp. 990- 997 ,(2005) , 10.1104/PP.105.061408
David Gresham, Douglas M Ruderfer, Stephen C Pratt, Joseph Schacherer, Maitreya J Dunham, David Botstein, Leonid Kruglyak, Genome-wide detection of polymorphisms at nucleotide resolution with a single DNA microarray. Science. ,vol. 311, pp. 1932- 1936 ,(2006) , 10.1126/SCIENCE.1123726
R. W. Michelmore, I. Paran, R. V. Kesseli, Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 88, pp. 9828- 9832 ,(1991) , 10.1073/PNAS.88.21.9828
Lars M. Steinmetz, Himanshu Sinha, Dan R. Richards, Jamie I. Spiegelman, Peter J. Oefner, John H. McCusker, Ronald W. Davis, Dissecting the architecture of a quantitative trait locus in yeast Nature. ,vol. 416, pp. 326- 330 ,(2002) , 10.1038/416326A
Timothy P. Ellis, Kevin G. Helfenbein, Alexander Tzagoloff, Carol L. Dieckmann, Aep3p stabilizes the mitochondrial bicistronic mRNA encoding subunits 6 and 8 of the H+-translocating ATP synthase of Saccharomyces cerevisiae Journal of Biological Chemistry. ,vol. 279, pp. 15728- 15733 ,(2004) , 10.1074/JBC.M314162200
Adam M Deutschbauer, Ronald W Davis, Quantitative trait loci mapped to single-nucleotide resolution in yeast Nature Genetics. ,vol. 37, pp. 1333- 1340 ,(2005) , 10.1038/NG1674
Charlotte Paquin, Julian Adams, Isolation of Sets of a, α, a/α, a/a and α/α isogenic strains in Saccharomyces cerevisiae. Current Genetics. ,vol. 6, pp. 21- 24 ,(1982) , 10.1007/BF00397636
Rachel B Brem, Gaël Yvert, Rebecca Clinton, Leonid Kruglyak, Genetic dissection of transcriptional regulation in budding yeast. Science. ,vol. 296, pp. 752- 755 ,(2002) , 10.1126/SCIENCE.1069516
Haoping Liu, Cora Ann Styles, Gerald R. Fink, Saccharomyces cerevisiae S288C has a mutation in FLO8, a gene required for filamentous growth. Genetics. ,vol. 144, pp. 967- 978 ,(1996) , 10.1093/GENETICS/144.3.967
E. A. WINZELER, B. LEE, J. H. McCUSKER, R. W. DAVIS, Whole genome genetic-typing in yeast using high-density oligonucleotide arrays. Parasitology. ,vol. 118, pp. 73- 80 ,(1999) , 10.1017/S0031182099004047