Reactive Oxygen Species-Mediated DNA Damage and Apoptosis in Human Skin Epidermal Cells After Exposure to Nickel Nanoparticles
作者： Saud Alarifi , Daoud Ali , Saad Alakhtani , Entissar S. Al Suhaibani , Ahmed A. Al-Qahtani
DOI: 10.1007/S12011-013-9871-9
关键词:
摘要: Nickel nanoparticles (NiNPs) are increasingly used in various applications due to their unique properties. However, there is little information concerning the toxicity of NiNPs human skin cell (A431). The present study was designed investigate cytotoxicity, apoptosis, and DNA damage A431 cells. A cellular proliferative capacity test showed that induce significant cytotoxicity a dose- time-dependent manner. were also found oxidative stress evidenced by generation reactive oxygen species (ROS) depletion glutathione (GSH). Further, co-treatment with antioxidant N-acetylcysteine (NAC) mitigated ROS NiNPs, suggesting potential mechanism stress. induced elevation lipid peroxidation, catalase, superoxide dismutase caspase-3 activity In addition, NAC suppressed NiNP-induced activity. fragmentation analysis using comet assay cause genotoxicity Therefore, points out capability resulting apoptosis genotoxicity. This warrants more careful assessment before industrial applications.
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