Extracellular polymeric substance from Aphanizomenon flos-aquae induces apoptosis via the mitochondrial pathway in A431 human epidermoid carcinoma cells.
作者： XING XUE , YING LV , QING LIU , XIAOLAN ZHANG , YOUHONG ZHAO
关键词:
摘要: Extracellular polymeric substance (EPS) is a secreted during algal growth, which has been found to have numerous health-promoting effects. In the present study, A431 human epidermoid carcinoma cells were selected as target and cultivated in vitro an experimental model investigate anti-cancer effect of extracellular substances from Aphanizomenon flos-aquae (EPS-A) possible underlying mechanism. Apoptosis- cell cycle-associated molecules well mitochondrial membrane potential quantified using flow cytometry (FCM). FCM showed that EPS-A induced cycle arrest, led loss function increase necrotic late apoptotic cells. order evaluate apoptosis viability, acridine orange/ethidium bromide staining was used, morphological changes observed fluorescence microscopy typical characteristics observed. Following treatment with high dose EPS-A, transmission electron nuclear fragmentation, chromosome condensation, shrinkage expansion endoplasmic reticulum; bodies also conclusion, caused stimulated via pathway exhibited important activity.
spandidos-publications.net参考文章(22)
Xiaoying Zhang, Wei Chen, Ruth Guillermo, Gudiseva Chandrasekher, Radhey S Kaushik, Alan Young, Hesham Fahmy, Chandradhar Dwivedi, Alpha-santalol, a chemopreventive agent against skin cancer, causes G2/M cell cycle arrest in both p53-mutated human epidermoid carcinoma A431 cells and p53 wild-type human melanoma UACC-62 cells. BMC Research Notes. ,vol. 3, pp. 220- 220 ,(2010) , 10.1186/1756-0500-3-220
N.R. Monks, B. Li, S. Gunjan, D.T. Rogers, M. Kulshrestha, D.L. Falcone, J.M. Littleton, Natural Products Genomics: A novel approach for the discovery of anti-cancer therapeutics Journal of Pharmacological and Toxicological Methods. ,vol. 64, pp. 217- 225 ,(2011) , 10.1016/J.VASCN.2011.04.002
Meilan Xue, Yinlin Ge, Jinyu Zhang, Qing Wang, Lin Hou, Yongchao Liu, Lingling Sun, Quan Li, Anticancer Properties and Mechanisms of Fucoidan on Mouse Breast Cancer In Vitro and In Vivo PLoS ONE. ,vol. 7, pp. e43483- ,(2012) , 10.1371/JOURNAL.PONE.0043483
John J. Lemasters, Anna-Liisa Nieminen, Ting Qian, Lawrence C. Trost, Steven P. Elmore, Yoshiya Nishimura, Ruth A. Crowe, Wayne E. Cascio, Cynthia A. Bradham, David A. Brenner, Brian Herman, The mitochondrial permeability transition in cell death: a common mechanism in necrosis, apoptosis and autophagy. Biochimica et Biophysica Acta. ,vol. 1366, pp. 177- 196 ,(1998) , 10.1016/S0005-2728(98)00112-1
W. T. (PIM) DE NOBEL, HANS C. P. MATTHIJS, ERIC VON ELERT, LUUC R. MUR, Comparison of the light‐limited growth of the nitrogen‐fixing cyanobacteria Anabaena and Aphanizomenon New Phytologist. ,vol. 138, pp. 579- 587 ,(1998) , 10.1046/J.1469-8137.1998.00155.X
Saud Alarifi, Daoud Ali, Saad Alakhtani, Entissar S. Al Suhaibani, Ahmed A. Al-Qahtani, Reactive Oxygen Species-Mediated DNA Damage and Apoptosis in Human Skin Epidermal Cells After Exposure to Nickel Nanoparticles Biological Trace Element Research. ,vol. 157, pp. 84- 93 ,(2014) , 10.1007/S12011-013-9871-9
Delu Zhang, Chunxiang Hu, Gaohong Wang, Dunhai Li, Genbao Li, Yongding Liu, Zebrafish neurotoxicity from aphantoxins—cyanobacterial paralytic shellfish poisons (PSPs) from Aphanizomenon flos-aquae DC-1 Environmental Toxicology. ,vol. 28, pp. 239- 254 ,(2013) , 10.1002/TOX.20714
Jan-Hendrik Klock, Andrea Wieland, Richard Seifert, Walter Michaelis, Extracellular polymeric substances (EPS) from cyanobacterial mats: characterisation and isolation method optimisation Marine Biology. ,vol. 152, pp. 1077- 1085 ,(2007) , 10.1007/S00227-007-0754-5
Antonio Galfano, Giacomo Novara, Massimo Iafrate, Vincenzo De Marco, Marco Cosentino, Carolina D'Elia, Walter Artibani, Vincenzo Ficarra, Improvement of seminal parameters and pregnancy rates after antegrade sclerotherapy of internal spermatic veins Fertility and Sterility. ,vol. 91, pp. 1085- 1089 ,(2009) , 10.1016/J.FERTNSTERT.2008.01.040
Tiejun Zou, Xiang Liu, Shangshu Ding, Junping Xing, Evaluation of sperm mitochondrial function using rh123/PI dual fluorescent staining in asthenospermia and oligoasthenozoospermia Journal of Biomedical Research. ,vol. 24, pp. 404- 410 ,(2010) , 10.1016/S1674-8301(10)60054-1