Improved Induction of Antibodies against Key Neutralizing Epitopes by Human Immunodeficiency Virus Type 1 gp120 DNA Prime-Protein Boost Vaccination Compared to gp120 Protein-Only Vaccination

摘要: A major challenge in human immunodeficiency virus type 1 (HIV-1) vaccine development is to elicit potent and broadly neutralizing antibodies that are effective against primary viral isolates. Previously, we showed DNA prime-protein boost vaccination using HIV-1 gp120 antigens was more eliciting isolates than a recombinant protein-only approach. In the current study, analyzed difference antibody specificities rabbit sera elicited by these two immunization regimens peptide enzyme-linked immunosorbent assay competitive capture assay. Our results indicate regimen protein-alone approach inducing target key domains: V3 loop CD4 binding site. particular, positive targeting several peptides overlap with known area were detected only DNA-primed sera. Different profiles of provide insight into mechanisms behind elicitation better approach, our support use this further optimize Env formulations for HIV development.