Deletion of Chromosome 1p Loci and Microsatellite Instability in Neuroblastomas Analyzed with Short-Tandem Repeat Polymorphisms

摘要: We have analyzed DNA from 46 neuroblastoma tumors of all clinical stages and five ganglioneuroma together with corresponding control for loss heterozygosity (LOH) on the distal 1p chromosomal region (1p-LOH). The markers used analyses were genetically mapped polymorphisms detectable PCR analysis. In general, there was concordance among aggressive tumor stage, deletion, N- myc amplification, although exceptions found. Twelve (26%) displayed 1p-LOH, 11 being stage 4 1 2 (which progressed subsequently to 4), whereas 10 showed no 1p-LOH. Of 12 neuroblastomas shown had 8 cases it possible test parental origin chromosome involved in No significant correlation between LOH paternal or maternal allele Commonly deleted loci indicated that a tentative suppressor gene is defined proximally by marker D1S244 distally D1S80. One striking feature three one 3 presence alleles not present constitutional patients, i.e. , microsatellite instability. significance this phenomenon localized remains be clarified. Aggressive young children (younger than years age) seems homogenous disorder consistently showing concomitant 1p-LOH amplification. majority unfavorable older children, however, neither nor amplification could detected. This indicates biologically more heterogenous which genetic alterations other deletions also may contribute tumorigenesis.