Genetic heterogeneity and clonal evolution in neuroblastoma
作者： J Mora , N-K V Cheung , W L Gerald
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摘要: Tumour heterogeneity and clonal evolution at the genetic level may explain development of malignant or resistant disease during clinical progression neuroblastoma (NB). In this report we use 1p allelic analysis DNA ploidy to evaluate selection in vivo. We studied a total 69 tumours from 29 patients with NB. To tumour vivo used panel polymorphic markers mapping chromosome 1. 33 12 (group 1) were obtained different sites same surgery sequential surgeries without intervening chemotherapy heterogeneity. Paired samples 10 2) before after chemotherapy. 6 cases paired derived cell lines studied. Analysis changes by karyotype, FISH flow cytometry was performed 15 multiply recurred local-regional (LR) NB patients. Allelotype study revealed that 66% (8/12) group 1 heterogeneous, distinct patterns separated time location. 2 post-chemotherapy specimens 60% (6/10). showed pre-chemotherapy contained clones, one diploid triploid, whereas all tumor 100% diploid. These findings suggest exhibits high degree occurs course therapy progression. © 2001 Cancer Research Campaign http://www.bjcancer.com
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