Band 3 tyrosine kinase in avian erythrocyte plasma membrane is immunologically related to pp60c-src.
作者： D. Hillsgrove , C. G. Shores , J. C. Parker , P. F. Maness
DOI: 10.1152/AJPCELL.1987.253.2.C286
关键词:
摘要: We have identified in the plasma membrane of chicken erythrocyte a 60-kDa tyrosine-specific protein kinase immunologically related to transforming pp60v-src Rous sarcoma virus. The phosphorylated heavy chains tumor-bearing rabbit (TBR) antibodies reactive with pp60c-src at tyrosine immune complex assays. was as by [35S]methionine labeling erythrocytes and autophosphorylation complexes. migrated on two-dimensional gel electrophoresis an apparent pI molecular mass similar pp60c-src. A membrane-enriched fraction isolated from red cells contained majority activity. solubilized detergents 0.5% (wt/vol) Na-deoxycholate 1% (vol/vol) Nonidet P-40. One molar NaCl much less effective, indicating strong association membrane. Incubation [32P]ATP resulted phosphorylation anion transport band 3. Band 3 blocked TBR antibodies, that recognized responsible for phosphorylation. These results demonstrate avian contains tightly bound identical or closely this is vitro.
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