作者: W J Pinto , W R Nes
DOI: 10.1016/S0021-9258(18)32647-4
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摘要: When sterol biosynthesis in oxygen-deprived wild type Saccharomyces cerevisiae was prevented by the presence of 2,3-iminosqualene, an inhibitor 2,3-oxidosqualene cyclase, absolute requirement for a with 24 beta-methyl group found. Neither configuration nor size alkyl at C-24 could be altered. For instance, while beta-methylcholesterol (22-dihydrobrassicasterol) permitted good growth, contrary to earlier work without no growth all resulted from cholesterol or its alpha-methyl-, alpha-ethyl-, beta-ethyl derivatives (campesterol, sitosterol, and clionasterol, respectively). The only lacking which allowed desmosterol (24-dehydro-cholesterol), but metabolized C1-transfer reduction. supported absence inhibitor, small amounts endogenously synthesized beta-methylsterols (ergosterol 22-dihydroergosterol) were identified. This previously unrecognized specificity both chirality bulk suggests involvement protein binding least one roles plays this single-celled eukaryote.