Endobronchial eosinophils preferentially stimulate T helper cell type 2 responses.

摘要: Background:  Antigen-loaded eosinophils instilled intratracheally into mice were capable of migrating local lymph nodes and localize to the T cell-rich paracortical zones where they stimulated antigen-specific proliferation CD4+ cells. The aim present study was evaluate whether within tracheobronchial lumen can stimulate Th2 cell expansion by presenting antigen both in vitro vivo. Methods:  Airway recovered from ovalbumin-sensitized -challenged BALB/c mice, these then co-cultured with sensitized cells absence or presence anti-CD80 or/and -CD86 monoclonal antibodies. trachea mice. At 3 days thereafter, draining paratracheal removed teased suspensions for culture. Cell-free culture supernatants collected detection cytokines. Results:  Our data showed that airway functioned as CD80- CD86-dependent antigen-presenting (APCs) lymphocytes produce interleukin (IL)-4, IL-5, IL-13, but not interferon (IFN)-γ assay. When recipient migrated primed vivo IL-4, IFN-γ, production during also CD86-dependent. Conclusion:  Eosinophils lumina airways could process inhaled function APCs promote This investigation highlights potential only act terminal effector actively modulate immune responses amplifying responses.