Evaluation of a Microbiological Multi-Residue System on the detection of antibacterial substances in ewe milk

摘要: To protect both, public health and the dairy industry, from presence of antibiotic residues in milk, control programmes have been established, which include needed screening tests. This work focuses on application a Microbiological Multi-Residue System ewe method based use six different plates, each seeded with one following bacteria: Geobacillus stearothermophilus var. calidolactis (beta-lactams), Bacillus subtilis at pH 8.0 (aminoglycosides), Kocuria rhizophila (macrolides), Escherichia coli (quinolones), B. cereus (tetracyclines) 7.0 (sulphonamides), respectively. Twenty-three antimicrobial substances were analysed logistic regression was established for substance assayed to relate concentration zone microbial growth inhibition. Great linearity response observed (regression coefficients over 0.97). fact suggests possibility establishing decision level concentrations near Maximum Residue Limits (MRL). Zones inhibition suggested as proposed action levels groups (diameters 18 mm aminoglycoside, beta-lactam sulphonamide plates; 19 tetracycline plate, 21 macrolide 24 quinolone plate). Specificity cross-reactivity also assayed.