Polyhydroxybutyrate-co-hydroxyvalerate structures loaded with adipose stem cells promote skin healing with reduced scarring.

摘要: Currently available skin substitutes are still associated with a range of problems including poor engraftment resulting from deficient vascularization, and excessive scar formation, among others. Trying to overcome these issues, this work proposes the combination poly(3-hydroxybutyrate-co-hydroxyvalerate) (PHBV) structures adipose-derived stem cells (ASCs) offer biomechanical biochemical signaling cues necessary improve wound healing in full-thickness model. PHBV scaffold maintained moisture demonstrated enough mechanical properties withstand contraction. Also, exudate inflammatory cell infiltration enhanced degradation structure, thus progression. After 28 days all wounds were closed was completely degraded. The transplanted ASCs detected area only at day 7, correlating an up-regulation VEGF bFGF time point that consequently led significant higher vessel density group received loaded ASCs. Subsequently, dermis formed presence possesses more complex collagen structure. Additionally, anti-scarring effect observed indicated by down-regulation TGF-β1 α-SMA together increase TGF-β3, when These results indicate although able guide process reduced scarring, crucial enhance vascularization provide better quality neo-skin. Therefore, we can conclude bioactive scarring.