Comparison of individual component deletions in a glucose-specific phosphotransferase system revealed their different applications.
作者： Quanfeng Liang , Fengyu Zhang , Yikui Li , Xu Zhang , Jiaojiao Li
DOI: 10.1038/SREP13200
关键词:
摘要: The phosphoenolpyruvate-dependent glucose-specific phosphotransferase system (PTS(Glc)) is the main glucose uptake pathway in Escherichia coli that affects both substrate assimilation and metabolism leading to product formation. In this study, effect of single PTS(Glc) mutation on cell growth consumption was investigated by knocking out genes involved phosphotransfer cascade PTS(Glc). addition, distribution metabolites mutants analyzed. Each mutant confirmed have different adaptability presence xylose with ratios, a mixture high content can be completely consumed short time when ptsI employed. Finally, ptsH deletion for first applied succinate production due its well performance under anaerobic condition. Strain YL104H, which deleted, exhibited considerably increased yield aerobic conditions. titer overall productivity reached 511.11 mM 1.01 g/L/h after 60 h during whole-phase fermentation mineral salt medium. present results demonstrated co-utilization efficiency significantly improved if suitable selected.
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